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rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) antibody  (FUJIFILM)

 
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    FUJIFILM rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) antibody
    Rabbit Anti Ionized Calcium Binding Adapter Molecule 1 (Iba1) Antibody, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-ionized+calcium-binding+adapter+molecule+1+(iba1/rabbit+anti+iba1/pm39880314-68-86-97
    Average 90 stars, based on 1 article reviews
    rabbit anti-ionized calcium-binding adapter molecule 1 (iba1) antibody - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Comparison of the effects of two therapeutic strategies based on olfactory ensheathing cell transplantation and repetitive magnetic stimulation after spinal cord injury in female mice
    Article Snippet: The following primary antibodies were used: Rabbit anti‐platelet‐derived growth factor β (PDGFRβ, Abcam, ab32570, RRID:AB_777165), mouse anti‐glial fibrillary acidic protein (GFAP Cy3‐conjugated Sigma‐Aldrich, C9205, RRID:AB_476889), rabbit anti‐ionized calcium‐binding adapter molecule 1 (Iba1, Wako, 019‐19741, Osaka, Japan, RRID:AB_839504), and rat anti‐myelin basic protein (MBP, Millipore, MAB386, RRID:AB_94975).

    Article Title: Improved efficacy, tolerance, safety, and abuse liability profile of the combination of CR4056 and morphine over morphine alone in rodent models
    Article Snippet: The sections were blocked with 10% normal horse serum in PBS containing 0.3% Triton‐X for 90 min at room temperature and then incubated overnight at 4°C with rabbit anti‐ionized calcium‐binding adapter molecule 1 (Iba1) primary polyclonal antibody (1:350; Wako Cat# 019–19,741, RRID:AB_839504).

    Article Title: Neuronal gamma oscillations and activity-dependent potassium transients remain regular after depletion of microglia in postnatal cortex tissue.
    Article Snippet: TA B L E 1 List of antibodies used in the study Name Species Manufacturer/Order No/RRID Dilution Anti-Iba1 (ionized calcium-binding adapter molecule 1) Rabbit polyclonal Fujifilm-WAKO/019-19741/AB_839504 1:1,000 Anti-Parvalbumin (clone PARV-19) Mouse monoclonal Sigma-Aldrich/P3088/AB_477329 1:1,000 Anti-GFAP (glial fibrillary acidic protein) Rabbit polyclonal Agilent-Dako/Z0334/AB_10013382 1:1,000 Anti-MHC-II (MRC OX-6) Mouse monoclonal Abcam/ab23990/AB_447796 1:250 Cholinergic gamma oscillations were elicited by continuous application of acetylcholine and the acetylcholine-esterase inhibitor physostigmine via the recording solution (Kann et al., 2011; Ta et al., 2019).

    Article Title: Longitudinal autophagy profiling of the mammalian brain reveals sustained mitophagy throughout healthy aging
    Article Snippet: Rabbit polyclonal anti-Ionized calcium-binding adapter molecule 1 (IBA1) , FUJIFILM Wako Pure Chemical Corporation , Cat# 019-19741; RRID: AB_839504.

    Article Title: Intracerebral overexpression of miR-669c is protective in mouse ischemic stroke model by targeting MyD88 and inducing alternative microglial/macrophage activation.
    Article Snippet: The following primary antibodies were incubated overnight at room temperature (RT): rabbit anti-Iba1 (ionized calcium-binding adapter molecule 1, dilution 1:250; Wako PureChemical Industries Ltd., Tokyo, Japan), goat anti-Arg1 (dilution 1:300; Santa Cruz Biotechnology, Dallas, USA), rat anti-CD45 (leukocyte common antigen, dilution 1:200; Bio-Rad, Hercules, USA), and rat anti-MyD88 (dilution 1:100; R&D Systems, Minneapolis, USA) in 5% NGS or NRS.

    Article Title: Tumor-associated macrophages enhance tumor innervation and spinal cord repair
    Article Snippet: The following primary antibodies were used for 1, rat, 1:200; BD Biosciences, cat. #557355, Franklin Lakes, USA), anti-CD68 (rat, 1:200; Thermo Fischer Scientific, cat. #14-0681-82, Waltham, USA), anti-CD206 (goat, 1:400; R&D Systems, cat. #AF2535, Minneapolis, USA), anti-choline acetyltransferase (ChAT, goat, 1:200; Millipore, cat. #AB144P, Burlington, USA), collagen-I (Coll1, rabbit, 1.200; Abcam, cat. #AB34710, Cambridge, UK), collagen-III (Coll3, mouse, 1.200; GeneTex, cat. #GTX26310, Irvine, USA), anti-fibronectin (rabbit, 1:200; Dako, cat. #A0245, Nowy Sącz, Poland), anti-GFP (rabbit, 1:400; Thermo Fisher Scientific, cat. #A11122, Waltham, USA), anti-glial fibrillary acidic protein (GFAP, goat, 1:200; Abcam, cat. #AB53554, Cambridge, UK), anti-ionized-calcium binding adapter molecule-1 (Iba1, rabbit, 1:200; WAKO, cat. #019-19741, Osaka, Japan), anti-NeuN (mouse, 1:200; Millipore, cat. #MAB377, Burlington, USA), anti-neurofilament-200 (rabbit, 1:200; Sigma, cat. #N4142, St. Louis, USA), anti-peripherin (rabbit, 1:200; Millipore, cat. #AB1530), anti-RFP biotinylated antibody (rabbit, Rockland, cat. #600-401-379, Philadelphia, USA.).

    Article Title: Neuronal loss without amyloid‐β deposits in the thalamus and hippocampus in the late period after middle cerebral artery occlusion in cynomolgus monkeys
    Article Snippet: Double‐labeled immunofluorescence For double‐labeled immunofluorescence staining, sections were incubated with mixtures of rabbit and mouse primary antibodies overnight at 4°C: mouse anti‐Aβ 1‐16 (6E10), mouse anti‐Aβ 17‐24 (4G8), mouse anti‐Aβ 40 , mouse anti‐Aβ 42 , rabbit anti‐neuronal nuclei (NeuN; neuronal marker, 1:500, Millipore), rabbit anti‐ionized calcium‐binding adapter molecule 1 (Iba1; microglial marker, 1:500, Wako) or rabbit anti‐glial fibrillary acidic protein (GFAP; astrocytic marker, 1:500, Abcam).

    Incubation:

    Article Title: Regulation of neuronal lipid droplet aggregation by microglia through the lactate-lysosome signaling pathway
    Article Snippet: .. The 20-μm brain sections were rinsed with 0.01 M PBS, incubated for 1 h with 5% goat serum (Zhongshan Biotechnology Co., Beijing, China) supplemented with 0.3% Triton X-100 to block nonspeci c IgG binding, and then incubated overnight at 4°Cwith the following primary antibodies: rabbit anti-MAP2 (1:200; A nity Biosciences), mouse anti-neuronal nuclear antigen (NeuN, 1:500, Millipore, Billerica, MA, USA),rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1, 1:500, Wako Pure Chemical Industries, Ltd.), mouse anti-lysosome-associated membrane protein 2 (LAMP2, 1:200, Proteintech), mouse anticleaved caspase 1 (1:200, A nity Biosciences), mouse anti-caspase 1 (1:200, A nity Biosciences), and anti-LC3A/B (1:200, A nity Biosciences). .. Sections were then incubated with DyLight 549- or 488- conjugated secondary antibody (1:200, EarthOx SF, USA) for 2 h at room temperature.

    Blocking Assay:

    Article Title: Regulation of neuronal lipid droplet aggregation by microglia through the lactate-lysosome signaling pathway
    Article Snippet: .. The 20-μm brain sections were rinsed with 0.01 M PBS, incubated for 1 h with 5% goat serum (Zhongshan Biotechnology Co., Beijing, China) supplemented with 0.3% Triton X-100 to block nonspeci c IgG binding, and then incubated overnight at 4°Cwith the following primary antibodies: rabbit anti-MAP2 (1:200; A nity Biosciences), mouse anti-neuronal nuclear antigen (NeuN, 1:500, Millipore, Billerica, MA, USA),rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1, 1:500, Wako Pure Chemical Industries, Ltd.), mouse anti-lysosome-associated membrane protein 2 (LAMP2, 1:200, Proteintech), mouse anticleaved caspase 1 (1:200, A nity Biosciences), mouse anti-caspase 1 (1:200, A nity Biosciences), and anti-LC3A/B (1:200, A nity Biosciences). .. Sections were then incubated with DyLight 549- or 488- conjugated secondary antibody (1:200, EarthOx SF, USA) for 2 h at room temperature.

    Binding Assay:

    Article Title: Regulation of neuronal lipid droplet aggregation by microglia through the lactate-lysosome signaling pathway
    Article Snippet: .. The 20-μm brain sections were rinsed with 0.01 M PBS, incubated for 1 h with 5% goat serum (Zhongshan Biotechnology Co., Beijing, China) supplemented with 0.3% Triton X-100 to block nonspeci c IgG binding, and then incubated overnight at 4°Cwith the following primary antibodies: rabbit anti-MAP2 (1:200; A nity Biosciences), mouse anti-neuronal nuclear antigen (NeuN, 1:500, Millipore, Billerica, MA, USA),rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1, 1:500, Wako Pure Chemical Industries, Ltd.), mouse anti-lysosome-associated membrane protein 2 (LAMP2, 1:200, Proteintech), mouse anticleaved caspase 1 (1:200, A nity Biosciences), mouse anti-caspase 1 (1:200, A nity Biosciences), and anti-LC3A/B (1:200, A nity Biosciences). .. Sections were then incubated with DyLight 549- or 488- conjugated secondary antibody (1:200, EarthOx SF, USA) for 2 h at room temperature.

    Membrane:

    Article Title: Regulation of neuronal lipid droplet aggregation by microglia through the lactate-lysosome signaling pathway
    Article Snippet: .. The 20-μm brain sections were rinsed with 0.01 M PBS, incubated for 1 h with 5% goat serum (Zhongshan Biotechnology Co., Beijing, China) supplemented with 0.3% Triton X-100 to block nonspeci c IgG binding, and then incubated overnight at 4°Cwith the following primary antibodies: rabbit anti-MAP2 (1:200; A nity Biosciences), mouse anti-neuronal nuclear antigen (NeuN, 1:500, Millipore, Billerica, MA, USA),rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1, 1:500, Wako Pure Chemical Industries, Ltd.), mouse anti-lysosome-associated membrane protein 2 (LAMP2, 1:200, Proteintech), mouse anticleaved caspase 1 (1:200, A nity Biosciences), mouse anti-caspase 1 (1:200, A nity Biosciences), and anti-LC3A/B (1:200, A nity Biosciences). .. Sections were then incubated with DyLight 549- or 488- conjugated secondary antibody (1:200, EarthOx SF, USA) for 2 h at room temperature.



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